Human Pulmonary Artery Fibroblasts Search Results


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Human Pulmonary Artery Fibroblasts
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AcceGen Biotechnology human pulmonary artery adventitia fibroblasts
<t>Fibroblast</t> activation in 3D-bioprinted models of pulmonary arterial <t>adventitia.</t> A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.
Human Pulmonary Artery Adventitia Fibroblasts, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Human+Pulmonary+Artery+Fibroblasts/bio_rxiv__2022__11__11__516188-86-2-8?v=AcceGen+Biotechnology
Average 93 stars, based on 1 article reviews
human pulmonary artery adventitia fibroblasts - by Bioz Stars, 2026-08
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ScienCell human pulmonary adventitial fibroblasts hpaf
<t>Fibroblast</t> activation in 3D-bioprinted models of pulmonary arterial <t>adventitia.</t> A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.
Human Pulmonary Adventitial Fibroblasts Hpaf, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Human+Pulmonary+Artery+Fibroblasts/pmc02867379-60-0-5?v=ScienCell
Average 90 stars, based on 1 article reviews
human pulmonary adventitial fibroblasts hpaf - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Fibroblast activation in 3D-bioprinted models of pulmonary arterial adventitia. A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.

Journal: bioRxiv

Article Title: 3D-bioprinted, phototunable hydrogel models for studying adventitial fibroblast activation in pulmonary arterial hypertension

doi: 10.1101/2022.11.11.516188

Figure Lengend Snippet: Fibroblast activation in 3D-bioprinted models of pulmonary arterial adventitia. A) Fibrotic activation in soft and stiffened 3D hydrogels measured by αSMA expression. HPAAFs in stiffened constructs were significantly more positive for αSMA than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. B) Representative confocal images of immunostaining for αSMA, actin, and DAPI in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent αSMA immunofluorescence than cells in soft constructs. Scale bar = 250 µm. C) Fibroblast proliferation in soft and stiffened 3D bioprinted constructs measured by EdU positivity. HPAAFs in stiffened constructs were significantly more positive for EdU than cells in soft constructs. Columns represent mean ± SEM, n = 3. *, p < 0.05, Mann-Whitney U test. D) Representative confocal images of immunostaining for EdU and Hoechst dye in soft and stiffened 3D hydrogels. HPAAFs in stiffened constructs showed more prevalent EdU immunofluorescence than cells in soft constructs. Scale bar = 300 µm.

Article Snippet: [ ] Human pulmonary artery adventitia fibroblasts (HPAAFs; Accegen, Fairfield, NJ) from a 2-year-old male patient were cultured in complete HPAAF culture media according to manufacturer instructions.

Techniques: Activation Assay, Expressing, Construct, MANN-WHITNEY, Immunostaining, Immunofluorescence